PRECISE pregnancy cohort: challenges and strategies in setting up a biorepository in sub-Saharan Africa

Table 4 Summary of sample types collected in PRECISE

Sample type	Collection tube	Aliquots	Storage
Blood	EDTA	Blood spot	Room temperature
	EDTA	Whole blood	−80 °C
	EDTA	Buffy coat	−80 °C
	EDTA	Plasma	−80 °C
	Serum	Serum	−80 °C
Urine	no treatment	Urine	−80 °C
Urine	no treatment	Urine sediment	−80 °C
Vaginal swab	Copan eSwab	TE buffer	−80 °C
Vaginal swab	Dacron swab	Protease inhibitor	−80 °C
Placenta, fetal membranes and cord	No treatment	Peripheral biopsies	Snap frozen
		Central biopsies	Snap frozen
		Peripheral biopsies	Paraffin embedding
		Central biopsies	Paraffin embedding
		Cord tissue	Snap frozen
		Cord tissue	Paraffin embedding
		Membrane	Paraffin embedding
Heel prick	No treatment	Blood spot	Room temperature
Neonatal stool	DNA/RNA Shield tube	Swab	−80 °C

ISSN: 1742-4755